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KMID : 0377519870120030393
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Chung-Ang Journal of Medicine
1987 Volume.12 No. 3 p.393 ~ p.408
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Determination of an Adequate Dose of Isoproterenol to Produce an Infarct-like Lesion on the Heart
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Suh, Young-Dong/서영동
Kim, Ho-dirk/Rah, Bong-jin/김호덕/라봉진
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Abstract
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Since it has been found that in experimental animals, especially in rats, injections of large and repeated doses of ISO produce patches of necrosis in the myocardium, it has widely been used at a dose from tens to hundreds miligrams per kilogram of body weight. Light microscopic, electron microscopic and morphometric studies were under-taken to determine an adequate dose of ISO to produce an infarct-like lesion on the heart in rat model. The morphologic effects were examined at 24 hours following two subcutaneous injections of ISO at various doses between 20 mg and 400 mg/kg.
The NIV F considerably increased at a dose between 40 mg and 150 mg/kg (p<0.01), while it acutely decreased at a dose between 200 mg and 400 mg/kg (p<0.01). Three types of cardiac necrosis could be seen, i.e., moderate ischemic injury at a dose´ between 4o mg and 100 mg/kg severe ischemic injury at a dose bet-een 100 and 150 mg/kg, and irreversible ischemic injury at a dose between 200 mg and 400 mg/ kg. A dose of ISO between 40 mg and 60 mg/kg could be regarded as a standard subcutaneous dose and a `point of no return´ Iesion could be produced at a dose between 150 mg and 200 mg/kg.
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